2026
A perfusable decellularized plant-based air-liquid-interface-on-a-chip for investigating inorganic dust aerosol exposure.
This study reports the effects of inhaled dust aerosols on pulmonary barriers, along with offering a plant-based, biocompatible, and tissue-emulating air–liquid interface on-a-chip model. The developed model offers a versatile platform for studying toxicity of diverse particles and a preclinical evaluation of respiratory therapeutics.
2025
Dual role of electrical stimulation and a biomimetic matrix in neural differentiation within a microfluidic platform
Utku Devamoglu,Yagmur Arslan,Leila Sabour-Takanlou,Cigir Biray-Avci&Ozlem Yeşil-Çeliktaş
Herein, a biomimetic microfluidic platform was developed to promote neural differentiation of stem cells by recapitulating physicochemical and physicomechanical factors in the neural microenvironment. In order to address this, the supportive role of electrical stimulation (ES) was assessed under various conditions by using immunofluorescence staining of mesenchymal stromal cell markers (CD45, CD90), the neuroepithelial stem cell protein marker (Nestin) and the microtubule-associated protein 2 marker (MAP2). Moreover, the combinational effect of ES and a cell-derived matrix (CDM), or a three-dimensional tissue-derived matrix (TDM), was explored.
2024
ICU patient-on-a-chip emulating orchestration of mast cells and cerebral organoids in neuroinflammation
Pelin Saglam-Metiner, Sena Yanasik, Yusuf Caglar Odabasi, Jennifer Modamio, Moritz Negwer, Cigir Biray-Avci, Ayse Guler, Ali Erturk, Ender Yildirim & Ozlem Yesil-Celiktas
This study highlighted a newly designed ICU patient-on-a-chip platform that is consisted of a vascular chamber with a membrane lined up hCMEC/D3 to recapitulate the BBB, and a neural tissue formed by co-culturing of hiPSCs differentiated mast cells and cerebral organoids in 3D matrix. We hypothesize that the administration of propofol and midazolam for specific durations to the on-chip platform allows investigation of the alterations in BBB and the cellular response in neural tissue of ICU patients.
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2023
Spatio-temporal dynamics enhance cellular diversity, neuronal function and further maturation of human cerebral organoids
Pelin Saglam-Metiner, Utku Devamoglu, Yagmur Filiz, Soheil Akbari, Goze Beceren, Bakiye Goker, Burcu Yaldiz, Sena Yanasik, Cigir Biray Avci, Esra Erdal, Ozlem Yesil-Celiktas
We demonstrate two different engineering approaches, the rotating cell culture system (RCCS) microgravity bioreactor and a newly designed microfluidic platform (µ-platform) to improve harvestability, reproducibility and the survival of high-quality cerebral organoids and compare with those of traditional spinner and shaker systems.
2022
Supercritical carbon dioxide dried double layer laponite XLS and alginate / polyacrylamide construct and immune response
Yagmur Filiz, PelinSaglam-Metiner, Seymanur Ersoy, OzlemYesil-Celiktas
In this study, a double layer hydrogel was successfully synthesized, where alginate/polyacrylamide were formulated to represent cartilage layer (5–10 % (w/w) total polymer ratio) and laponite XLS (2−5-8% (w/w))/alginate/polyacrylamide formed bone layer.
2021
An alginate-poly (acrylamide) hydrogel with TGF-β3 loaded nanoparticles for cartilage repair: Biodegradability, biocompatibility and protein adsorption
Ecem Saygili, Elif Kaya, Esra Ilhan-Ayisigi, Pelin Saglam-Metiner, Emine Alarcin, Aslihan Kazana, Ezgi Girgic, Yong-Woo Kim, Kasim Gunes, Guler Gamze Eren-Ozcan, Dilek Akakin, Jeong-Yun Sun, OzlemYesil-Celiktas
Here, we report a holistic and interdisciplinary approach for cartilage tissue engineering to design DN hydrogel-based cartilage implants by using Alg and PAAm polymer networks.
2020
A hepatocellular carcinoma–bone metastasis-on-a-chip model for studying thymoquinone-loaded anticancer nanoparticles
Fatemeh Sharifi, Ozlem Yesil-Celiktas, Aslihan Kazan, Sushila Maharjan, Saghi Saghazadeh, Keikhosrow Firoozbakhsh, Bahar Firoozabadi & Yu Shrike Zhang
HCC–bone metastasis-on-a-chip platform was demonstrated to model certain key aspects of the cancer metastasis process, hence corroborating the potential of enabling investigations on metastasis-associated biology as well as improved anti-metastatic drug screening.
2019
A foreign body response‐on‐a‐chip platform
Fatemeh Sharifi,Su Su Htwe,Martina Righi,Hua Liu,Anna Pietralunga,Ozlem Yesil-Celiktas,Sushila Maharjan,Byung-Hyun Cha,Su Ryon Shin,Mehmet Remzi Dokmeci,Nihal Engin Vrana,Amir M. Ghaemmaghami,Ali Khademhosseini,Yu Shrike Zhang
Here, we report the development of a physiologically relevant and microscopy-friendly in vitro microfluidic platform, the FBR-on-a-chip (FBROC), to reproduce the dynamic effects of circulating immune cells on the implant occurring in FBR.
2018
Advances in glioblastoma multiforme treatment: new models for nanoparticle therapy
In this paper, we critically summarize current glioblastoma therapy, with a focus on emerging nanomedicine and therapies that capitalize on cell-specific signaling in glioblastoma. We follow this summary by discussing computational modeling approaches focused on optimizing these emerging nanotherapeutics for brain cancer.
Ege University
Department of Bioengineering
35040 Bornova-Izmir/TURKEY